Biotechnological potential of aquatic plant ecdysteroids
DOI:
https://doi.org/10.51258/RJH.2025.07Keywords:
aquatic plants, Lemna minor, ecdysteroidsAbstract
Ecdysteroids are polyhydroxylated sterols initially identified in insects but later reported in numerous terrestrial plants, including Ajuga, Rhaponticum, and Cyanotis. These metabolites display diverse biological properties such as anabolic, adaptogenic, antioxidant, antimicrobial, and antitumor effects, making them highly relevant for pharmaceutical and nutraceutical applications. However, their occurrence in aquatic plants remains poorly studied. This review highlights the potential of fast-growing macrophytes, particularly Lemna minor (duckweed), as promising sources of ecdysteroids and other bioactive compounds including flavonoids and amino acids. Available literature is critically analyzed to compare existing findings and to assess their implications for food technology, pharmacy, and biotechnology. By integrating current evidence, this article positions Lemna minor as a model system for exploring aquatic plant-based metabolites. The review also outlines future research priorities and industrial opportunities for sustainable production of nutraceutical and pharmaceutical agents from aquatic sources.
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Alharthi, F., Althagafi, H. A., Jafri, I., Oyouni, A. A. A., Althaqafi, M. M., Al-Hijab, L. Y. A., Al-Hazmi, N. E., Elagib, S. M., and Naguib, D. M. (2024). Phytochemical Composition and Bioactivities of Some Hydrophytes: Antioxidant, Antiparasitic, Antibacterial, and Anticancer Properties and Mechanisms. Plants, 13(15), 2148. https://doi.org/10.3390/plants13152148
Bethancourt-Dalmasí, H., Viuda-Martos, M., Lucas-González, R., Borrás, F., & Fernández-López, J. (2025). Exploring the Applications of Lemna minor in Animal Feed: A Review Assisted by Artificial Intelligence. Applied Sciences, 15(12), 6732. https://doi.org/10.3390/app15126732
Dinan, L, and Lafont, R. (2022). Chapter Two - Ecdysteroids as defensive chemicals, Editor(s): Russell Jurenka, Advances in Insect Physiology, Academic Press, Volume 63: 107-154, ISSN 0065-2806, ISBN 9780323952644, https://doi.org/10.1016/bs.aiip.2022.09.001.
Guibout, L., Mamadalieva, N., Balducci, C., Girault, J. P., & Lafont, R. (2015). The minor ecdysteroids from Ajuga turkestanica. Phytochemical analysis : PCA, 26(5), 293–300. https://doi.org/10.1002/pca.2563
Hunyadi, A., Herke, I., Lengyel, K. 2016. Ecdysteroid-containing food supplements from Cyanotis arachnoidea on the European market: evidence for spinach product counterfeiting. Sci Rep 6, 37322 https://doi.org/10.1038/srep37322
Kumpun, S., Girault, J.-P., Dinan, L., Blais, C., Maria, A., Dauphin-Villemant, C., Yingyongnarongkul, B., Suksamrarn, A., & Lafont, R. (2011). The metabolism of 20-hydroxyecdysone in mice: Relevance to pharmacological effects and gene switch applications of ecdysteroids. Journal of Steroid Biochemistry and Molecular Biology, 126(1–2), 1–9.
Mamadalieva, N. Z., Hussain, H., Mollica, A., Zengin, G., Mamadalieva, R. Z., Elhady, S. S., Fadil, S. A., Ashour, M. L., & Youssef, F. S. (2022). Ecdysteroids as Potent Enzyme Inhibitors and Verification of Their Activity Using In Vitro and In Silico Docking Studies. Life (Basel, Switzerland), 12(6), 824. https://doi.org/10.3390/life12060824
Roumanille, R., Vernus, B., Brioche, T., Descossy, V., Van Ba, C. T., Campredon, S., Philippe, A. G., Delobel, P., Bertrand-Gaday, C., Chopard, A., Bonnieu, A., Py, G., & Fança-Berthon, P. (2020). Acute and chronic effects of Rhaponticum carthamoides and Rhodiola rosea extracts supplementation coupled to resistance exercise on muscle protein synthesis and mechanical power in rats. Journal of the International Society of Sports Nutrition, 17(1), 58. https://doi.org/10.1186/s12970-020-00390-5
Seferli, M., Kotanidou, C., Lefkaki, M., Adamantidi, T., Panoutsopoulou, E., Finos, M. A., Krey, G., Kamidis, N., Stamatis, N., Anastasiadou, C., and Tsoupras, A. (2024). Bioactives of the Freshwater Aquatic Plants, Nelumbo nucifera and Lemna minor, for Functional Foods, Cosmetics and Pharmaceutical Applications, with Antioxidant, Anti-Inflammatory and Antithrombotic Health Promoting Properties. Applied Sciences, 14(15), 6634. https://doi.org/10.3390/app14156634
Thiem, B., Kikowska, M., Maliński, M. P., Kruszka, D., Napierała, M. & Florek, (2017). Ecdysteroids: production in plant in vitro cultures. Phytochemistry Reviews, 16(4), 603–622. https://doi.org/10.1007/s11101-016-9483-z
Todorova, V., Ivanova, S., Chakarov, D., Kraev, K., & Ivanov, K. (2024). Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition. Nutrients, 16(9), 1382. https://doi.org/10.3390/nu16091382
Ullah, H., Gul, B., Khan, H. et al. Effect of growth medium nitrogen and phosphorus on nutritional composition of Lemna minor (an alternative fish and poultry feed). BMC Plant Biol 22, 214 (2022). https://doi.org/10.1186/s12870-022-03600-1
Varun, S. T., Prasad, R. M. V., Jayalaxmi, P., and Sandhya Rani, M. S. (2021). A study on nutrient analysis of Azolla pinnata. Indian Journal of Animal Production and Management, 37(1-4), 102-106. https://doi.org/10.48165/ijapm..37.1-4.15
Van-Dyck, I. Vanhoudt, N., Vives, J., Batlle, I., Horemans, N., Nauts, R., Van Gompel, A., Claesen, Vangronsveld, J., (2021). Effects of environmental parameters on Lemna minor growth: An integrated experimental and modelling approach, Journal of Environmental Management, Volume 300, 2021, 113705, ISSN 0301-4797, https://doi.org/10.1016/j.jenvman.2021.113705.
Zhou, Y., Stepanenko, A., Kishchenko, O., Xu, J., & Borisjuk, N. (2023). Duckweeds for Phytoremediation of Polluted Water. Plants (Basel, Switzerland), 12(3), 589. https://doi.org/10.3390/plants12030589
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